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. Author manuscript; available in PMC: 2009 Aug 31.
Published in final edited form as: Cold Spring Harb Symp Quant Biol. 2008 Nov 6;73:33–38. doi: 10.1101/sqb.2008.73.016

Figure 2. Mutation of the CPE in the Spin 4.1 kb 3'UTR leads to ineffective translation.

Figure 2

Top panel – schematic representation of the Spin 4.1 kb reporter constructs used to determine the effect of mutation of the CPE on message stability in the FGO and ovulated oocyte. The CPE sequences in the Spin 4.1 kb transcript are indicated next to “intact 3'UTR”. The 3'CPE is 37 nt from the PAS, and the 5'CPE 119 nt away from the PAS. The sequences replacing the 3'CPE sequence in the Spin 4.1 kb transcript are indicated next to “mutation in CPE”. PAS indicates the polyadenylation signal sequence. Lower panel – Graphic representation of the decrease in translation of the reporter construct when the CPE sequence in the Spin 4.1 kb transcript is mutated. FGO – full-grown oocyte; FGO -> OO – transition from FGO to ovulated oocyte; OO – ovulated oocyte. Inline graphic Graph of reporter construct containing the intact CPE sequence; Inline graphic Graph of reporter construct containing the mutated CPE sequence.