Skip to main content
. Author manuscript; available in PMC: 2009 Aug 31.
Published in final edited form as: Cell. 2008 Dec 26;135(7):1299–1310. doi: 10.1016/j.cell.2008.12.006

Figure 2. Gene-Specific Histone and DNA Methylation Profiles in Rat ES Cells and REFs.

Figure 2

(A and B) The enrichment of H3K4me3 and H3k27me3 at the TSSs of indicated genes was determined by ChIP qPCR in DAc2 rat ES cells (A) and in REFs derived from E14.5 DA rat embryos (B). Data are the average of triplicate qPCR results.

(C) Expression levels of indicated genes in rat ES cells (ESC) and REFs were determined by qRT-PCR and were normalized to Gapdh. Data are the average of triplicate samples.

(D) Bisulfite sequencing of DNA methylation of the Oct4 and Nanog promoter regions in rat ES cells and REFs. Arrows indicate the transcriptional start sites. Unmethylated and methylated CpGs are shown with open and filled circles, respectively.