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. Author manuscript; available in PMC: 2009 Aug 31.
Published in final edited form as: Development. 2008 Jun 11;135(14):2435–2444. doi: 10.1242/dev.014704

Figure 6.

Figure 6

Morphant dorsal axial cells at St. 13 exhibit a dramatic dose-dependent reduction in adhesion to recombinant extracellular domain of C-cadherin (A). However, cell surface levels of C-Cad are unchanged in morphant cells, because similar levels of control and morphant cell C-Cad is available on the cell surface for trypsin digestion (B). Morphant cells also show a dramatic reduction in adhesion to fibronectin (FN) (C), and integrin α5 surface levels also are not affected by myosin IIB depletion (D). Failure of blastopore closure due to MHC-B depletion is not rescued by exogenous expression of C-Cad, consistent with the hypothesis that C-Cad levels are not limiting in morphant embryos (E). Adhesion to FN in morphant cells is rescued by co-expression of exogenous human MHC-B (F).