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. 2009 Jul 16;28(16):2437–2448. doi: 10.1038/emboj.2009.194

Figure 1.

Figure 1

Exogenous octopamine and dopamine treatments of cre∷gfp animals. Animals carrying cre∷gfp were placed on agar plates containing (A) no amine, (B) 3 mg/ml octopamine, (C) 1 mg/ml dopamine, or (D) 3 mg/ml octopamine plus 1 mg/ml dopamine. Fluorescence images were taken after 6 h of incubation. GFP expression was observed in the SIA neurons in octopamine-treated animals and it was decreased by dopamine. White dotted lines outline the head of each animal. Scale bars, 20 μm. (E) The number of GFP-expressing SIA neurons per animal was determined after cre∷gfp animals were incubated on plates containing no amine (NA), octopamine (OA), dopamine (DA), or octopamine plus dopamine (O+D) for 6 h. Error bars indicate the standard errors of the means. At least 80 animals were tested. *P<0.001 (Tukey–Kramer multiple comparison test), compared with OA.