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. 2009 Jul 23;28(16):2414–2427. doi: 10.1038/emboj.2009.196

Figure 4.

Figure 4

Loss of A-type lamins impacts genomic stability. (A) Quantification of the percentage of metaphases with signal-free ends in the different lines of Lmna+/+ (1–4) and Lmna−/− (A–E) MEFs. Note the threefold increase in signal-free ends in Lmna−/− MEFs. (B) Quantification of the percentage of metaphases with chromosome and/or chromatid breaks. A twofold increase in breaks was observed in Lmna−/− lines. (C) Quantification of the percentage of cells presenting >5 γH2AX-labelled DNA repair foci. Note the increase in basal damage in Lmna−/− MEFs. Graphs show results from at least three independent experiments. Bars represent standard error. (D) Karyotype analysis of more than 100 metaphases shows a clear increase in the number of metaphases with an aberrant number of chromosomes in Lmna−/− MEFs. (E) Quantification of number and percentage of Lmna+/+ and Lmna−/− MEFs presenting more than two centrosomes by labelling with anti-γ tubulin antibody. *Represents P-value of statistical significance.