Table 3.

Differences in deuterium levels for peptides derived from liganded BirA relative to the unliganded protein.

	ΔDeuterium
Peptide	biotin	btnSA	btnOH	bioAMP
001–010	+0.9	−0.4	0.0	−0.7
014–050	+1.5	−0.5	−1.4	−2.6
051–066	−0.2	−2.5	−2.9	−3.5
085–101	−6.2	−10.6	−10.4	−11.3
095–101	+0.5	−0.8	−0.3	−0.5
136–146	+0.5	−0.5	−0.7	−1.6
136–148	+0.8	−1.5	−1.0	−2.1
137–146	+0.4	0.0	−0.2	−0.9
178–188	−2.0	−2.6	−2.8	−3.5
180–188	−2.4	−2.8	−3.0	−3.2
201–209	+2.1	0.0	+0.9	0.0
242–248	−0.2	+0.2	+0.2	+0.2
243–252	−0.2	−0.7	−0.7	−0.9
253–265	+1.5	+0.7	+0.7	+0.4
266–278	0.0	−0.5	−0.3	−1.3
271–278	−0.7	−0.4	−0.4	−0.4
272–278	−0.5	−0.5	−0.5	−0.5
279–298	+0.5	−0.3	0.0	−1.3
279–299	+0.0	−0.9	−0.5	−1.7
287–299	−2.6	−3.1	−2.4	−3.1
300–313	+2.6	+0.9	+1.3	−0.7
300–316	+2.5	+1.0	+0.6	−0.6
300–321	+3.5	+1.7	+1.8	−0.3

Values were calculated by subtracting the value found for the peptide in the liganded protein from that found for that in the unliganded. Results for the shaded peptides are shown in Fig. 6.