Table 2.

Table 2A. Mitochondrial respiratory complexes I–IV
Parameter (nM/mg protein/s)	Control	TNF	TNF+ APO	TNF+ Temp
Complex I activity	11.90 ± 1.21	3.91 ± 0.65^*	4.96 ± 0.46	8.51 ± 0.99^‡,^§
Complex II activity	17.70 ± 0.90	9.34 ± 0.5^*	10.49 ± 0.96	15.17 ± 0.94^‡,^§
Complex III activity	22.61 ± 1.08	12.68 ± 1.69^*	12.37 ± 0.74	18.25 ± 1.97^‡,^§
Complex IV activity	22.05 ± 0.7	16.74 ± 0.98^*	16.50 ± 1.8	20.81 ± 0.85
Table 2B. Stimulatory effect of glutamate on mitochondrial respiratory complex I–enzyme activity
Parameter (nM/mg protein/s)	Control	TNF	TNF+ APO	TNF+ Temp

Basal	8.23 ± 0.21	3.21 ± 0.22s^*	3.14 ± 0.21^†	7.12 ± 0.4^‡,^§
Glutamate	12.67 ± 0.75	5.02 ± 0.08^*	6.37 ± 0.11	12.15 ± 0.28^‡,^§
Glutamate+ rotenone	15.55 ± 0.73	8.79 ± 0.49^*	11.69 ± 1.44	17.53 ± 1.49^‡,^§
Glutamate+ rotenone+ SOD	24.90 ± 2.70	13.77 ± 0.54^*	20.10 ± 0.99	30.32 ± 1.32^‡,^§
Table 2C. Mitochondrial respiratory complex I–superoxide production
Parameter (nM/mg protein/s)	Control	TNF	TNF+ APO	TNF+ Temp

Basal	5.24 ± 0.19	8.16 ± 0.17^*	6.72 ± 0.06	4.37± 0.16^‡,^§
Glutamate	3.72 ± 0.08	7.63 ± 0.12^*	7.47 ± 0.11	4.03 ± 0.22^‡,^§
Glutamate+ rotenone	5.73 ± 0.20	11.59 ± 0.36^*	8.73 ± 0.39	4.46 ± 0.29^‡,^§
Glutamate+ rotenone+ SOD	3.71 ± 0.18	7.13 ± 0.12^*	7.27 ± 0.21	4.19 ± 0.20^‡,^§

Enzyme activity of mitochondrial respiratory complexes I–IV. Mitochondria were probed with CMH and one of the following substrates was added: 20 mM glutamate (complex I), 5 mM succinate (complex II), 5 mM pyruvate (complex III), or 15 μM cytochrome c (complex IV). Values are expressed as means ± SEM.

Mitochondrial complex I activity under inhibitory and stimulatory conditions. Values are expressed as means ± SEM.

p< 0.05, control vs TNF.

^†

p< 0.05, TNF vs TNF+ APO.

^‡

p< 0.05, TNF vs TNF+ Temp.

^§

p< 0.05, TNF+ APO vs TNF+ Temp.

Mitochondrial complex I superoxide production. Values are expressed as means ± SEM.