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. Author manuscript; available in PMC: 2010 Jul 15.
Published in final edited form as: J Immunol. 2009 Jun 26;183(2):1301–1312. doi: 10.4049/jimmunol.0803567

Figure 3. Efficient induction of IFNγ-dependent effector mechanisms in Mtb-infected macIL-10tg mice.

Figure 3

FVB (open symbols) and macIL-10tg (solid symbols) mice were infected with 100 CFU Mtb via the aerosol route. Gene-expression of IFNγ-dependent (a) Nos2 and (b) LRG-47 (lrgm1) was determined in lung homogenates from uninfected and mice infected for 21, 49 and 89 days by RTPCR based on expression of Hprt. Data represent means and standard deviations of 3 mice. One experiment representative of two performed is shown. (c) For immunohistological detection of NOS2, 2−3 μm sections were prepared from FVB and macIL-10tg mice 42 days after aerosol infection and staining was performed with a polyclonal rabbit anti-mouse NOS2 antibody. Representative results of 4 mice per group obtained in three independent experiments are shown.