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. Author manuscript; available in PMC: 2009 Dec 1.
Published in final edited form as: Exp Gerontol. 2008 Aug 30;43(12):1086–1093. doi: 10.1016/j.exger.2008.08.050

Figure 3.

Figure 3

PrP expression is downregulated in CR mice and SIRT1 knockout mice. (A) PrP mRNA in brain and liver and (B) PrP protein levels in brain are downregulated in CR mice and SIRT1 KO mice. PrP mRNA and protein expression levels were compared between WT mice and SIRT1 KO mice fed AL, or WT mice fed AL or calorie restricted. mRNA levels were quantified by real time PCR and relative abundance of mRNA was obtained by normalization to cyclophilin levels (n=4). Protein levels were quantified by western blotting with anti-PrP antibody SAF83. Both tubulin and actin were used as loading controls. (C) SIRT1 promotes the expression of the PrP promoter. 293T cells were transfected with a luciferase reporter driven by the PrP promoter, with or without addition of a SIRT1 construct and/or resveratrol. The expression from the reporter construct was quantified by a luciferase assay.