Time course of mecamylamine to the hypothermic effect of 3-IC. Mice were injected intraperitoneally with 0.2 mg/kg 3-IC and one group of animals was pre-injected with MEC 1 mg/kg 10 minutes before 3-IC or saline. The time course of hypothermia was registered for DBA/2J (A) (n=3) and C57BL/6J (n=3) (B) mice. Maximal effect on body temperature for each treatment is shown for both mice strains (C) (* p<0.05, Student's t test, comparison to 3-IC alone) Time course of 3-IC-induced hypothermia and the effect of hexamethonium was tested, mice were pre-injected with hexamethonium (HXMT, 10 mg/kg) 10 minutes before 0.2 mg/kg 3-IC or saline and body temperature registered in DBA/2J (n=4) (D) and C57BL/6J mice (n=5) (E). Comparison of maximal hypothermia for each treatment in both mice strains is showed (F) (* p<0.05, Student's t test, comparison to 3-IC alone). The effect of scopolamine on 3-IC-induced hypothermia was tested injecting animals intraperitoneally with 10 mg/kg scopolamine 10 minutes before 0.2 mg/kg 3-IC or saline and body temperature was recording at time intervals for DBA/2J (n=4) (G) and C57BL/6J (n=4) (H). The maximal effect on body temperature for each treatment in both mice strains was plotted (I).