Table 2. Autoradiographic values for subsets of [125I]epibatidine binding.
Mice were chronically intravenously infused with 3-IC 0.2 mg/kg/h for 7 days. After chronic 3-IC treatment, the brains were removed and series of coronal slices were obtained. Subsets of [125I]epibatidine binding were calculated from binding of [125I]epibatidine 200 pM in absence or presence of cytisine 50 nM. Non-specific binding was determined by adding 100 μM nicotine. (* p<0.05, ** p<0.01, *** p<0.001, Student's t test, n=3 for saline and 3-IC treatment)
| Cytisine-sensitive [125I]epibatidine binding (fmol/mg) | Cytisine-resistant [125I]epibatidine binding (fmol/mg) | |||||
|---|---|---|---|---|---|---|
| Saline | 3-iodocytisine | % change | Saline | 3-iodocytisine | % change | |
| cingulate cortex | 16.6 ± 3.3 | 23.6 ± 1.5 | 42.0 | 0.99 ± 0.12 | 1.25 ± 0.13 | 26.9 |
| parietal cortex (layers 1-3) | 5.3 ± 1.1 | 9.1 ± 1.0 | 71.7 * | 0.50 ± 0.01 | 0.62 ± 0.06 | 24.6 |
| parietal cortex (layers 4-6) | 10.6 ± 2.0 | 19.0 ± 1.6 | 79.7 * | 0.89 ± 0.03 | 1.29 ± 0.07 | 45.9 |
| retrosplenial Cortex | 19.6 ± 2.8 | 26.6 ± 2.7 | 35.6 | 1.20 ± 0.05 | 1.63 ± 0.11 | 35.8 |
| anterior olfactory nucleus | 10.5 ± 5.1 | 16.1 ± 1.4 | 53.5 | 17.07 ± 1.30 | 13.27 ± 2.48 | -33.3 |
| hippocampus (CA1-3) | 5.7 ± 0.3 | 9.0 ± 0.2 | 56.3 *** | 0.81 ± 0.06 | 0.98 ± 0.06 | 20.2 |
| dentate gyrus (granular layer) | 8.4 ± 0.9 | 12.3 ± 0.6 | 46.8 * | 0.98 ± 0.05 | 1.15 ± 0.06 | 17.3 |
| subiculum | 32.6 ± 9.1 | 41.7 ± 3.8 | 27.8 | 0.98 ± 0.05 | 1.89 ± 0.10 | 3.0 |
| caudate putamen | 12.1 ± 1.3 | 20.3 ± 1.9 | 68.3 * | 1.84 ± 0.08 | 2.39 ± 0.29 | 18.2 |
| nucleus accumbens | 8.3 ± 1.7 | 15.9 ± 2.1 | 91.8 * | 2.02 ± 0.02 | 1.95 ± 0.21 | 12.9 |
| substantia nigra | 31.6 ± 1.8 | 43.6 ± 7.3 | 38.0 | 1.5 ± 0.1 | 1.6 ± 0.2 | 6.7 |
| ventral tegmental area | 17.3 ± 1.6 | 25.1 ± 1.5 | 45.2 * | 2.82 ± 0.46 | 3.4 ± 0.69 | 20.5 |
| zona incerta | 8.8 ± 0.7 | 13.7 ± 1.2 | 55.3 * | 1.73 ± 0.18 | 2.34 ± 0.22 | 5.2 |
| olfactory tubercle | 7.9 ± 0.3 | 14.5 ± 1.6 | 83.5 * | 3.5 ± 0.3 | 2.9 ± 0.5 | -17.1 |
| optic tract | 6.7 ± 0.6 | 15.1 ± 2.9 | 126.2 * | 1.39 ± 0.05 | 1.75 ± 0.22 | 26.1 |
| anteromedial thalamic nucleus | 50.7 ± 7.5 | 50.3 ± 4.0 | -0.8 | 3.26 ± 0.17 | 3.97 ± 0.25 | 22.0 |
| dorsomedial thalamic nucleus | 55.4 ± 10.6 | 52.9 ± 5.2 | -4.5 | 3.59 ± 0.10 | 3.78 ± 0.50 | 5.3 |
| ventromedial thalamic nucleus | 51.1 ± 3.4 | 50.7 ± 1.5 | -0.8 | 2.90 ± 0.59 | 3.29 ± 0.46 | 13.4 |
| medial habenula | 112.3 ± 31.7 | 171.3 ± 36.4 | 52.6 | 326.93 ± 28.78 | 282.84 ± 31.53 | -13.5 |
| fasciculus retroflexus | 39.8 ± 4.5 | 40.2 ± 4.0 | 1.0 | 61.57 ± 2.35 | 63.47 ± 4.86 | 3.1 |
| dorsolateral geniculate nucleus | 64.5 ± 5.8 | 70.7 ± 3.9 | 9.7 | 10.17 ± 1.26 | 9.89 ± 0.66 | -2.8 |
| medial geniculate nucleus | 38.9 ± 0.7 | 49.4 ± 8.2 | 27.2 | 3.21 ± 0.45 | 4.43 ± 0.80 | 38.1 |
| inferior colliculus (dorsal) | 13.5 ± 4.2 | 22.8 ± 2.1 | 68.7 * | 6.06 ± 1.45 | 10.17 ± 2.62 | 67.7 |
| inferior colliculus (central) | 6.1 ± 1.1 | 12.2 ± 2.5 | 97.2 * | 2.38 ± 0.36 | 4.00 ± 1.42 | 68.2 |
| inferior colliculus (external) | 8.9 ± 1.3 | 16.8 ± 2.1 | 88.9 * | 2.06 ± 0.20 | 2.55 ±0.19 | 24.2 |
| interpeduncular nucleus | 196.0 ±38.4 | 194.7 ± 42.8 | -0.6 | 244.14 ± 31.95 | 211.95 ± 31.83 | 13.2 |
| superior colliculus (sup. gray) | 34.1 ± 1.0 | 51.2 ± 4.4 | 50.2 ** | 10.16 ±1.26 | 11.25 ± 0.12 | 10.8 |
| superior colliculus (optic nerve) | 29.5 ± 0.5 | 47.3 ± 2.2 | 60.6 *** | 3.19 ± 0.25 | 3.21 ± 0.14 | 0.5 |