Fig. 5.

Methylation analysis of HMF5 (Xa21G). (A) Genomic organization of the Xa21 locus. A 4 kb genomic locus is illustrated with the exons (box) and introns (line) indicated. Exons (E) are numbered in Arabic letters starting from the transcription initiation site (+1 which corresponds to nucleotide position 25 208 591 of chromosome 2). CCGG sites are indicated by vertical bars over the sequence, and mapping and probed regions are indicated by horizontal bars. The regions for direct methylation mapping are located at nucleotide positions –369 to + 144 (region I), + 484 to + 808 (region II) and + 2043 to + 2413 (region III). (B) Phylogenetic relationship between Xa21G and other Xa21-related proteins. The proteins compared are D (U72726), B (Xa21) (U37133·1), C (U72723), A1 (U72725), E (U72724), A2 (U72727), F (U72728) and G (NM-001053967). (C) Inheritance of demethylation. Aliquots of 15 µg of genomic DNA isolated from the wild type (WT) and the indicated progeny (F₃–F₉) of Line-2 plants were digested with either HpaII (H) or MspI (M), and analysed by DNA blot hybridization using a unique 1 kb fragment (positions 329–1341) as the probe.