Figure 2.

Effects of m.o.i. on reporter mRNA and protein levels. A) Cardiocytes were infected with increasing m.o.i. of c-jun/βGal adenovirus and electrically stimulated to contract at 1 Hz for 48 h. c-jun/βGal mRNA levels are expressed as the fold increase, which was calculated by setting the βGal RNA level measured at the lowest m.o.i. to a value of 1. B) Corresponding measurements of GFP mRNA and GFP fluorescence. C) Summary data showing relation between GFP mRNA and c-jun/βGal mRNA levels. Values are means ± se, n = 8 experiments.