Figure 1.

15d-PGJ₂ abrogates stimulation of collagen gene expression. Foreskin fibroblasts transiently transfected with 772COL1A2/CAT reporter construct were preincubated with 15d-PGJ₂ (10 μM) for 60 min, followed by TGF-β2 (12.5 ng/ml) for a further 48 h. At the end of incubation, cell lysates were assayed for CAT and luciferase activities. Transfection efficiency was monitored by Renilla luciferase assays. Results shown represent means ± sd of triplicate determinations. Open bars denote untreated fibroblasts; solid bars denote TGF-β-treated fibroblasts. *P < 0.001. Bottom panel: culture supernatants were subjected to Western blot analysis using anti-type I collagen antibody.