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15d-PGJ2 abrogates induction of p300-Smad2/3 interaction. Following pretreatment with 15d-PGJ2 for 60 min, fibroblasts were incubated with TGF-β for 60 min. Whole-cell lysates were immunoprecipitated with antibodies to p300 and subjected to Western blot analysis. Representative immunoblots are shown.
