Figure 7.
Ectopic p300 rescues stimulation of COL1A2 activity in the presence of 15d-PGJ2. Confluent fibroblasts transiently cotransfected with increasing concentration of p300 or HAT-deleted mutant p300 (p300ΔHAT) expression vectors along with 772COL1A2/CAT were pretreated with 15d-PGJ2 for 60 min. Following further incubation with TGF-β2 (12.5 ng/ml) for 24 h, cell lysates were assayed for their CAT and luciferase activities. Transfection efficiency was monitored by Renilla luciferase assays. Results represent means ± sd of triplicate determinations. Open bars denote untreated fibroblasts; solid bars denote TGF-β-treated fibroblasts.