Figure 11.
Dogs vaccinated with Na-APR-1mut generate antibodies that neutralize enzymatic activity of Na-APR-1wt and have higher mean Hb concentrations than control dogs after heterologous challenge with A. caninum. A) Hb concentrations of individual vaccinated and control dogs immediately prior to necropsy. Highlighted area between 12 and 14 g/dl Hb denotes normal range in a healthy dog. Horizontal lines denote median values. B) Na-APR-1wt (250 ng) was incubated with different amounts of dog anti-Na-APR-1mut IgG or control dog IgG for 2 h at 37°C in the presence of methoxycoumarin-4-acetyl-GKPILFFRLK(DNP)-d-Arg-Amide. Data are expressed as relative fluorescence generated by cleavage of the substrate in the presence of dog IgGs and are presented as means of 3 reproducible triplicate experiments.