Whole cell protein extracts were prepared from HT-AR1 cells grown in DHT-containing media for the indicated times and separated by 7.5 or 12.5% SDS-PAGE. A, differential expression of RhoB, PTGFβ, caveolin-2, and Egr-1 proteins in DHT-treated HT-AR1 cells. The RhoB antibody detects both the unmodified and post-translationally modified (RhoB*) forms of the protein (50, 51). B, expression levels of integrin α6, gp96, plakoglobin, and N-cadherin proteins are not altered by DHT treatment in HT-AR1 cells under these same conditions.