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. Author manuscript; available in PMC: 2009 Aug 31.
Published in final edited form as: J Biol Chem. 2003 Oct 23;279(2):937–944. doi: 10.1074/jbc.M311325200

Fig. 7. Transient transfection of HT-AR1 cells with a constitutively active form of RhoB induces growth arrest.

Fig. 7

A, transient transfection of HT-AR1 cells with the pCDNA3.1 vector alone, or the same vector encoding HA-tagged activated HA-RhoBact (G14), or dominant negative HA-RhoBdn (T19N) variant, alters cell proliferation. Transfected cells were cultured in the presence or absence of 10 nM DHT for 3 days and viable cells were counted by trypan blue exclusion. B, representative results of HA immunostaining of transiently transfected HT-AR1 cells from the same experiment as in A. Results show that DHT-induced cytoskeletal reorganization was not affected by ectopic expression of RhoBact or RhoBdn. The HA immunostaining showed that >60% of the HT-AR1 cells were HA positive 3 days after transfection (data not shown).