Fig. 2.

Cross-presentation with equivalent antigen uptake. (a) To account for antigen uptake in the evaluation of CD8⁺T cell responses, OVA–FITC was conjugated onto polystyrene beads of varying sizes and incubated with DC2.4 at varying concentrations. Uptake was assessed by flow cytometry and quantified as the geometric mean fluorescence intensity of FITC. (b) OVA conjugated to 3 μm, 500 nm, and 50 nm beads was delivered at a concentration of 10 μg/ml and the CD8⁺T cell response was assessed.