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. Author manuscript; available in PMC: 2009 Aug 31.
Published in final edited form as: Biochem J. 2009 May 27;420(3):439–449. doi: 10.1042/BJ20090214

Figure 3. CcO Vb silencing caused a reduction in the steady state levels of other CcO subunits.

Figure 3

A,B. 50 μg (mitochondrial extract) or 80 μg (whole cell extract) protein from control and VbKD1 cells were subjected to immunoblot analysis using monoclonal antibodies for CcO subunits I, II, IV, Va, and Vb, and either TOM 20 for mitochondrial samples or β-actin for whole cell extract as loading controls. C,D. The protein levels for various subunits were quantitated as described in Figure 1A. E. The mRNA levels for indicated CcO subunits were determined using Real Time-PCR. The mRNA levels were normalized to β-actin and the level of each subunit present in control was arbitrarily set at one.