Effect of GM-CSF on expression of MHC II and costimulatory molecules by eosinophils isolated from spleens of IL-5 Tg mice. Fresh eosinophils (green) or eosinophils incubated with GM-CSF (10 ng/ml) for 24 h (red) were stained with FITC-labeled anti-MHC II, anti-CD40, anti-CD80 anti-CD86, or isotype control IgG (gray) mAbs. Flow cytometric data, with cell counts on the y-axis and fluorescence intensity on the x-axis, are representative of three experiments.