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. Author manuscript; available in PMC: 2009 Aug 31.
Published in final edited form as: J Neurochem. 2008 Apr 12;106(2):541–550. doi: 10.1111/j.1471-4159.2008.05411.x

Figure 8.

Figure 8

Mild hypothermia prevents FasL cleavage by inhibiting MMPs. Cultured neurons were exposed to oxygen glucose deprivation (OGD) under normothermic (37C) or mild hypothermic (33C) conditions. Some cultures were treated with the pan-MMP inhibitor GM6001. Control cultures were kept in balanced salt solution containing 5.5 mM glucose (5.5). A. Western blots of control or OGD exposed cultures of the soluble fraction (sFasL) showed less sFasL under conditions of mild hypothermia, but more membrane bound FasL (mFasL). Cultures treated with GM6001 at 37C were no different from cultures treated at 33C. Cultures exposed to OGD at 33C and treated with inhibitor had further increases in mFasL and decreases in sFasL. B. Western blot data are shown as the ratio of relative optical densities of the soluble to membrane fractions. Results are shown from 3 experiments performed in triplicate from cells prepared from 3 separate dissections. (33, 37=culture temperature; 5.5=control wash; +=GM6001; +=no GM6001; *P<0.05, **P<0.001)