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. 2009 Sep 1;20(17):3810–3817. doi: 10.1091/mbc.E08-12-1202

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© 2009 by The American Society for Cell Biology

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Figure 7. — Proportion of C2C12 cells that internalize epitope tags after transfection. (A) FLAG uptake is dependent upon the transfected IGF-I construct. There was no difference in the proportion of FLAG-positive cells after transfection of FLAG-IGF-IA or FLAG-IGF-IB. Expression of mature IGF-I (FLAG-IGF-IStop) significantly reduced the proportion of FLAG-positive cells. Mutation of the primary cleavage site between mature IGF and the EA-peptide in IGF-IA (AK68G) also resulted in a significant decrease in FLAG-positive cells. (B) HA uptake is independent of the transfected IGF construct. There was no significant difference in the proportion of HA-positive cells in the presence or absence of the sequence encoding mature IGF. Mutation of the primary cleavage sites between IGF and the E-peptide (AK68G, BK68G) did not affect the proportion of HA-positive cells. Comparisons by one-way ANOVA followed by Tukey's multiple comparison test.