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Ipl1 localization and function are not altered in the absence of Mcm21. (A) Chromosome spreads were performed on nocodazole-arrested WT and mcm21Δ cells (SBY8352, SBY8353) to localize Cse4 and Ipl1-GFP. (B) Ipl1-FLAG was immunoprecipitated from untagged, WT, and mcm21Δ cultures (SBY3, SBY7018, and SBY7019). The immunoprecipitates were immunoblotted with anti-Flag (left) or subjected to in vitro kinase assays (right).
