Figure 2.

Atg26 is not essential for pexophagy of small methanol-induced peroxisomes. (A and B) The WT (GS115), Δatg26 (PDG3d), and Δatg1 (R12) cells were induced in methanol medium for (A) 0.5 h or (B) 1.5 h and transferred to ethanol medium. At the indicated time points culture samples were collected and immunoblotted with antibodies against peroxisomal AOX. (C and D) The WT (STN26), Δatg26 (STN28), and Δatg1 (STN29) cells were induced in methanol medium for (C) 0.5 h or (D) 1.5 h and transferred to ethanol medium for 12 h. Pexophagy, as judged by a reduction in peroxisome number per cell and/or by the vacuolar delivery of GFP-SKL, was monitored by fluorescence microscopy. Peroxisomes were labeled with GFP-SKL and vacuolar membranes with FM 4-64. Bar, 5 μm.