Skip to main content
. 2009 Sep 1;20(17):3918–3929. doi: 10.1091/mbc.E09-03-0187

Figure 3.

Figure 3.

Disruption of actin remodeling impairs delivery of GLUT4 to the PM. (A) HA-GLUT4–expressing adipocytes were serum starved for 120 min and treated with DMSO ± 10 μM Lat-B for 1 h. Cells were then either unstimulated (Bas) or stimulated with 100 nM insulin (Ins) for 20 min at 37°C, fixed, and incubated with anti-HA antibodies in the absence of saponin. Representative images are shown. Scale bar, 60 μm. (B) IRAP-TfR–expressing cells were serum starved for 120 min and treated with DMSO ± 10 μM Lat-B for 60 min. Cells were then either unstimulated (Bas) or stimulated with 100 nM insulin (Ins) for 20 min at 37°C. IRAP-TfR on the PM was visualized by surface labeling with Tf-Alexa-488 at 4°C for 60 min. Representative images are shown. Scale bar, 40 μm. (C) As in A, but HA-GLUT4–expressing cells were grown in 96-well plates, and surface HA-GLUT4 expression under nonpermeabilizing conditions was expressed as a percentage of total HA-GLUT4 determined under permeabilizing conditions. * p < 0.05 versus DMSO control insulin (n = 3 experiments).