Figure 6.

HeLa and HCT116 cancer cells do not completely inhibit replication fork progression in response to Cdc6 deficiency. Dual-labeled (green, CldU and red, IdU) DNA fibers were generated for asynchronously growing HeLa (A) or HCT116 (B) cells treated with indicated siRNA(s). Representative labeled DNA fibers and percentage of total labeled DNA fibers for new firing origins (NFO) or progressing forks (PF) for each condition are shown. We counted ≥950 labeled DNA fibers from three independent experiments for each condition. Lengths of all counted replication forks were measured as described in Figure 4, B and C.