Fig. 2.

Expression analysis of patient specific DiPS cells. (A) Semiquantitative analysis of expression of OCT4, SOX2, REX1, NANOG, KLF4, GDF3, and β ACTIN. Control PCR (no RT) is included. (B) Hierarchical cluster analysis of different DiPS, HUES and fibroblast lines. (C) Quantitative assessment of viral transgene expression (tgOCT4, tgSOX2, and tgKLF4) levels. Viral transgene expression was normalized to control infected BJ fibroblasts (isolation occurred 7 days post infection). Uninfected HUES and fibroblast lines were used as controls. The experiment was performed in duplicates and the error bars represent SD.