Skip to main content
. 2009 Aug 31;106(38):16357–16362. doi: 10.1073/pnas.0907996106

Fig. 2.

Fig. 2.

Effect of endotoxin on alveolar fluid clearance and inflammation. Instillation of endotoxin into the lung lobe was associated with a significant decrease in (A) alveolar fluid clearance (AFC) and (B) an increase in inflammatory cytokine secretion. (A) The decrease in AFC in the endotoxin-injured lung lobe was dependent on the presence of fresh whole human blood in the perfusate. AFC in the endotoxin-injured lung lobe with fresh whole blood was significantly decreased compared to control AFC at 0 or 4 h measurements without endotoxin or to experiments with endotoxin without blood in the perfusate at 4 h. AFC was measured by the change in protein concentration of a 5% albumin instillate in the lung lobe over 1 h and expressed as mean AFC (%/h per 150 mL alveolar fluid) ± SD. For each condition, n = 3–6; *, P < 0.0001 vs. control AFC (0 h), †, P < 0.0013 vs. control AFC (4 h) by ANOVA (Bonferroni). (B) The addition of endotoxin into the lung lobe was associated with a significant increase in IL-1β, TNFα, and IL-8 levels in the alveolar fluid compared to the control lobe at 0 or 4 h. n = 3–6; *, P < 0.0001 vs. control (0 h), †, P < 0.0001 vs. control (4 h) for IL-1β, TNFα, and IL-8 by ANOVA (Bonferroni).