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. 2009 Aug 31;106(38):16357–16362. doi: 10.1073/pnas.0907996106

Fig. 6.

Fig. 6.

Effect of MSC on net fluid transport among primary cultures of human alveolar epithelial type II cells (A). Human alveolar epithelial type II cells were exposed to cytomix with and without MSC grown in the bottom chamber for 24 h. Cytomix reduced net fluid transport among the type II cells. The presence of allogeneic MSCs pretreated with a non-specific, non-targeting negative control siRNA partially restored the decrease in net fluid transport induced by cytomix. Pretreatment of MSCs with a KGF siRNA reduced the protective effect of MSCs. n = 3; *, P < 0.003 vs. control lobe by ANOVA (Bonferroni). Changes in the apical membrane and total cellular protein levels of the major sodium transport protein, αENaC, by human alveolar epithelial type II cells (B). Human alveolar epithelial type II cells exposed to cytomix with or without MSCs in the bottom chamber for 24 h. Total cellular protein or apical membrane protein levels, biotinylated using the Cell Membrane Isolation Kit (Pierce), were isolated. αENaC protein levels were measured by Western blot and expressed as the percent protein levels of control ± SD of each sample, measured in triplicates. *, P < 0.02 compared to controls for biotinylated proteins; *, P < 0.008 compared to controls for total protein by ANOVA (Bonferroni). A representative Western blot is depicted above each figure.