Fig. 5.
Model for Ty1 CNC. Gag and Gag-Pol precursor proteins, Ty1AS RNA, Ty1 mRNA, and tRNA-Meti (tM) are shown during VLP assembly and protein maturation. In CNC- VLPs lacking Ty1AS RNAs, efficient VLP assembly, protein processing, and reverse transcription occurs. Ty1 mRNA/AS RNA base paring may be required for copackaging into CNC+ VLPs. Ty1AS RNAs may inhibit several steps in the process of retrotransposition such as blocking the cleavage of the Pol precursor protein by PR, destabilizing IN and RT, or preventing tRNA-Meti annealing with the mRNA.