Table 3.

Thermal denaturation parameters of wild type and mutant HγS-crystallin

protein	ΔSma	ΔHm(kcal/mol)b	Tmc	ΔTmd	Δ(ΔG)e
WT (Fl Ratio curve)	0.462±0.014	161.057±4.940	75.56±0.15
MUT (Fl Ratio curve)	0.099 ± 0.001	33.231±0.379	61.49±0.17	-14.07	-6.498
N↔I
MUT (Fl Ratio curve)	0.318±0.012	108.963±4.064	69.45±0.25	-6.11	-2.822
I↔U
WT (CD curve)	0.440±0.018	153.2±6.269	75.05±0.16
MUT(CD curve)	0.219±0.036	74.2±12.192	65.46±0.15	-9.59	-4.219

^aslope of ΔG versus T at Tm in Kcal/mol/deg.

^bΔHm=[Tm(K)]×( ΔSm) in Kcal/mol.

^cmidpoint of thermal unfolding curve in °C.

^ddifference between the Tm values.

^eΔ(ΔG)= ΔTm ×ΔSm. where ΔSm is value for the wild type protein.