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. Author manuscript; available in PMC: 2009 Dec 15.
Published in final edited form as: Anal Chem. 2008 Dec 15;80(24):9630–9634. doi: 10.1021/ac8009559

Table 1. Aptamer surface density as a function of thrombin binding and efficiency for SAMs formed with different thymidine linkers.

Entry Thiol-linked aptamer Surface density of aptamer in the mixed SAM (pmol•cm-2)a Maximum thrombin bound to the mixed SAM (pmol•cm-2) Thrombin/Aptamer ratio at saturation (%)
1 5′-C6-T0-A1 25.3 2.96 11.7
2 5′-C6-T5-A1 21.0 4.10 19.5
3 5′-C6-T10-A1 18.2 3.89 21.4
4 5′-C6-T20-A1 12.23 3.39 27.7
5 5′-C6EG6-T0-A1 25.5 4.70 18.6
6 5′-C6EG6-T5-A1 21.5 6.49 30.2
7 5′-C6EG6-T10-A1 17.62 5.36 30.4
8 5′-C6EG6-T20-A1 11.95 4.82 40.4
a

Maximum standard deviation from duplicate values was ± 0.03 pmol•cm-2.