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. Author manuscript; available in PMC: 2009 Dec 15.
Published in final edited form as: Anal Chem. 2008 Dec 15;80(24):9630–9634. doi: 10.1021/ac8009559

Table 2. The amount of thrombin bound to C6-T1-A-3′ and 5′-C6-T1-A monolayers of comparable surface density.

Entry Aptamer Surface density of aptamer (pmol•cm-2)a Maximum specific thrombin bound to the mixed SAM (pmol•cm-2)
1 5′-HS(CH2)6-T-A1-3′ 24.7 3.2
2 3′-HS(CH2)6-T-A1-5′ 41.0 1.3
3 3′-HS(CH2)6-T-A1-5′ 21.3 3.4
a

Maximum standard deviation from duplicate values was ± 0.03 pmol•cm-2.