Table 2. The amount of thrombin bound to C6-T1-A-3′ and 5′-C6-T1-A monolayers of comparable surface density.
Entry | Aptamer | Surface density of aptamer (pmol•cm-2)a | Maximum specific thrombin bound to the mixed SAM (pmol•cm-2) |
---|---|---|---|
1 | 5′-HS(CH2)6-T-A1-3′ | 24.7 | 3.2 |
2 | 3′-HS(CH2)6-T-A1-5′ | 41.0 | 1.3 |
3 | 3′-HS(CH2)6-T-A1-5′ | 21.3 | 3.4 |
Maximum standard deviation from duplicate values was ± 0.03 pmol•cm-2.