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H&E staining (A–C), Masson's trichrome staining (D–F, collagen stains blue), and EVG staining (G–I, elastin stains black) of the human umbilical arteries before (A, D, G) and after (B, E, H) incubation with CHAPS and SDS buffers for 22 h, respectively, and after further incubation with EGM-2 for 2 days (C, F, I). Original magnification, 20 ×; scale bar = 50 μm. Color images available online at www.liebertonline.com/ten.
