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. Author manuscript; available in PMC: 2010 Sep 15.
Published in final edited form as: Dev Biol. 2009 Jun 25;333(2):238–250. doi: 10.1016/j.ydbio.2009.06.020

Figure 6.

Figure 6

Simultaneous modulation of Stat3 and Mapk14 mimics miR-17 paralogs effect. (A) Real-time PCR was performed on total RNA extracted from MLE12 cells after transduction with Stat3 and Mapk14 adenovirus to determine the effectiveness of the adenoviral constructs. Data were compared to results from MLE12 cells transduced with a control adenovirus. Data are reported as means ± SEM. *=P ≤ 0.05. (B, 20X) IHC for CDH1 (red) and MAPK14 (green) was performed in MLE12 cells after the overexpression of Stat3 and Mapk14 by transduction with their specific adenovirus. (C) Immunoblotting from MLE12 cells’ whole extract after inhibition of Stat3 and Mapk14 by siRNA transfection was used to determine changes in CDH1.