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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Cell Signal. 2009 Jun 26;21(11):1569–1578. doi: 10.1016/j.cellsig.2009.06.003

Figure 2. FCPIA competition experiments to measure the affinity of LARG proteins for Gα13 subunits.

Figure 2

Competition of biotinylated RH/DH/PH binding to fluorescently labeled Gα13 with increasing amounts of unlabeled LARG RH/DH/PH (A) (WT or E354K) or (B) unlabeled LARG RH/DH/PH (WT or F1098D). Shown is one representative experiment. The error bars represent the SD of each data point measured in duplicate. The calculated Ki’s for RH/DH/PH-WT, -F1098D, and -E354K are 20 ± 4 nM (n=4), 20 ± 9 nM (n=3) and 540 ± 20 nM (n=2), respectively.