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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Cell Signal. 2009 Jun 26;21(11):1569–1578. doi: 10.1016/j.cellsig.2009.06.003

Figure 3. Mutation of the hydrophobic patch abrogates LARG-mediated transcription and stress fiber formation.

Figure 3

(A) Normalized luciferase activities of increasing amounts of HA-tagged LARG FL (WT and mutant) plasmids as measured in a luciferase reporter assay. Firefly luciferase activities were normalized to renilla activities, and reported as fold activation over the basal level. Data shown is reported as the mean ± SD and representative of three independent experiments, each measured in triplicate. Inset: the expression level of each protein was obtained by Western analysis and reported as percentage of WT level. Error bars are the SDs from three independent experiments, each measured in duplicate. (B) GFP fusions of LARG FL (WT, F1098D, I1100E, and I1109D) were transfected into NIH3T3 cells. Serum starved cells were then fixed and stained with Alexa fluor 594-Phalloidin to detect stress fibers. Only WT LARG was able to induce stress fiber formation. Scale bar: 10 µm.