Fig. 3.

Detection of 4-ONE-induced DNA degradation in HT-29 cells using fluorescence microscopy (a) and agarose gel electrophoresis (b). HT-29 cells were incubated with or without 10 µM 4-ONE and 20 ng/mL of TNF-α for 24 h at 37°C in a humidified atmosphere of 5% CO₂. Data are representative of at least three independent experiments.