Table 2. Predicted epitopes based on the HLA-B7 supertype motif and the clade A1 OLP library sequence used for epitope mapping.

U15119 position	Seqence	TAP binding scores*	ITOPIA HLA-B*0702 binding (%)**	Autologous Seq.***
8–16	YPCWWTWGI	2.893	none	no data
75–83	DPNPQEIYM	−0.901	none	DPNPQEIELDPNPREISLDPNPQEIPLDPNPQEVVL
114–22	KPCVQLTPL	2.175	64.2(----K----)	KPCVKLTPL KPCVQLTPLKPCVKLTPLKPCVKLTPL
205–13	CPKVTFEPI	−1.025	30.1	CPKVNFEPICPKVTFEPICPKVSFEPICPKVTFEPI
211–9	EPIPIRYCA	2.869	none	EPIPIHYCAEPIPIHYCAEPIPIHYCAEPIPIHYCA
213–21	IPIRYCAPA	0.198	58.5(---H-----)	IPIHYCAPAIPIHYCAPAIPIHYCAPAIPIHYCAPA
252–60	RPVVSTQLL	1.393	78.1	KPVVSTQLLKPVVSTQLLKPVVSTQLLKPVVSTQLL
408–16	LPCRIKQII	2.046	75.9	no data
489–497	APTKAKRRV	2.482	95.2	no data
843–51	IPRRIRQGL	3.59 (intermed)	180.2	no data

Only the last epitope (in bold) was recognized.

^*TAP binding scores were calculated using TAPpred, as per Ref. 20.

^**Percentage of Positive control binding. Where ITOPIA-tested sequences differed from OLP, these are noted.

^***Autologous sequences are from HLA B7 supertype+ subjects who did not recognize the epitopes listed (except IPRRIRQGL). Each sequence = 1 subject.