Example of real-time, multiplexed and specific DNA detection using the active CMOS biosensor array. Site A is functionalized with probe P1 and site B with P2. Target T1 is added, followed by T2 at a later time. Hybridization is observed at site A, where P1 hybridizes with fully-complementary T1, while no hybridization is seen at site B. After T2 is added, hybridization is observed at site B while the signal at site A remains constant. The extent of hybridization at sites A and B as a function of time is also shown.