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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1980 Sep;12(3):301–303. doi: 10.1128/jcm.12.3.301-303.1980

Evaluation of four methods for isolation of Neisseria gonorrhoeae.

B L Carlson, M S Haley, N A Tisei, W M McCormack
PMCID: PMC273579  PMID: 6783676

Abstract

Bio-Bag (Marion Laboratories, Kansas City, Mo.) type C is made up of a ziplock plastic bag which contains a Thayer-Martin plate and a crushable CO2-generating ampoule. This system was compared with the candle extinction jar, Gono-Pak (Nasco, Fort Atkinson, Wis.), and JEMBEC (GIBCO Diagnostics, Lawrence, Mass.) systems to determine their efficiency and reliability for the isolation of Neisseria gonorrhoeae. A total of 191 anal and 130 urethral specimens were tested. There were 104 isolates of N. gonorrhoeae (24 anal and 80 urethral). The candle jar and Bio-Bag systems each detected 98 (94%) of the isolates. The Gono-Pak and JEMBEC systems detected 102 (98%) and 100 (96%) of the 104 isolates, respectively. These differences are not statistically significant. The Bio-Bag has the advantage of immediate CO2 release as compared with the Gono-Pak and JEMBEC systems, where CO2 production is dependent on the release of moisture from the medium. The Bio-Bag is a useful system, especially in situations where it is not convenient to use a candle jar.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Holston J. L., Jr, Hosty T. S., Martin J. E., Jr Evaluation of the bag--CO2-generating-tablet method for isolation of Neisseria gonorrhoeae. Am J Clin Pathol. 1974 Oct;62(4):558–562. doi: 10.1093/ajcp/62.4.558. [DOI] [PubMed] [Google Scholar]
  2. Martin J. E., Armstrong J. H., Smith P. B. New system for cultivation of Neisseria gonorrhoeae. Appl Microbiol. 1974 Apr;27(4):802–805. doi: 10.1128/am.27.4.802-805.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Martin J. E., Jr, Jackson R. L. A biological environmental chamber for the culture of Neisseria gonorrhoeae. J Am Vener Dis Assoc. 1975 Sep;2(1):28–30. [PubMed] [Google Scholar]
  4. THAYER J. D., MARTIN J. E., Jr A SELECTIVE MEDIUM FOR THE CULTIVATION OF N. GONORRHOEAE AND N. MENINGITIDIS. Public Health Rep. 1964 Jan;79:49–57. [PMC free article] [PubMed] [Google Scholar]

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