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. 2009 Sep;11(9):921–933. doi: 10.1593/neo.09636

Figure 6.

Figure 6

LEDGF/p75 overexpression in tumors stimulates blood and lymphatic vessels' sprouting and induction of VEGF-C expression. Ex vivo analysis of subcutaneous tumors excised from mice inoculated with tumor cells in a dorsal skinfold window chamber (H1299 cells; A, B, E, F, I, J, M, and N) or in a tip of an ear (C6 cells; C, D, G, H, K, L, O, and P). Histologic sections stained with hematoxylin-eosin. (A and B) H1299 control and LEDGF-overexpressing tumors, respectively. (C and D) C6 control and LEDGF-overexpressing tumors. Scale bar, 200 µm. Immunohistochemical staining with anti-CD34 antibody of blood vessels in control H1299 and C6 (E and F, respectively) and LEDGF-overexpressing tumors (G and H, respectively; scale bar, 100 µm). Immunohistochemical staining of lymphatic endothelial cells using anti-LYVE-1 antibodies (H1299: control [I] and LEDGF-overexpressing tumors [J]; C6: control [K] and LEDGF-overexpressing tumors [L]; scale bar, 100 and 50 µm in the insert picture). In situ hybridization analysis using VEGF-C as a probe, indicating that VEGF-C expression is induced in tumors initiated from cells overexpressing LEDGF (H1299: control [M] and LEDGF overexpression [N]; C6: control [O] and LEDGF overexpression [P]; scale bar, 100 µm).