Figure 6.

Histologic diagnosis of NKs from mice treated with various doses of sunitinib. The contralateral left NKs (not bearing a tumor) resected from mice of the experiments described in Figure 4 were processed for histologic diagnosis, and kidney tissue sections were stained either with H&E (A) or with anti-CD31 immunostaining (B). NKs obtained from control mice showed multiple regular and thin vessels (V) by H&E and clear structures of vessels delineated by anti-CD31 staining of endothelial cells in vessel walls. After high SU40 dosage, dilatation of blood vessels was observed as seen by H&E. Enlarged vessels sometimes showed disruption of vessel walls seen by anti-CD31 staining. The milder effect of SU20 on normal vessels in NKs caused dilatation only in a few vessels, whereas most looked normal as seen by anti-CD31 staining. No effect on vessels in the NK was observed with SU10; the vessels looked thin and regular. Original magnifications, x40.