Figure 1.

Eye imaginal discs of third instar larvae with overexpression of activated Egfr and/or wild-type PI3K in glial cells. Immunofluorescence staining of (A–H) eye imaginal discs of third instar larvae. Nuclei of glial cells are red (α-Repo), glial cytoplasms are green (α-GFP), and photoreceptor neurons are blue (α-HRP). Images are projections of confocal image stacks. (A) Wild-type eye imaginal disc with optic stalk. (C, E, G) Ectopic gene expression resulted in a thickened optic stalk due to increased numbers of glial cells (arrowheads). (B) Wild-type eye imaginal disc with BN. (D, F, H) Ectopic gene expression resulted in overmigration of glial cells along BN (arrowheads).