Number of glial cells in different genotypes in eye imaginal discs of different age. Cell numbers expressing the glial marker repo were morphometrically determined in different developmental stages. Age of imaginal discs was grouped according to rows of photo-receptor neurons (0–5, 6–10, 11–15, 16–20 rows). In all investigated developmental stages, overexpression of λtop (activated Egfr) resulted in increased glial cell numbers. In later stages, overexpression of PI3K92E led to a significant decrease of cell numbers (*P < .05).