Figure 1. Transfusion of LR-PLT products induces MHC-matched BMT rejection.

(A) Experimental model of MHC-matched BMT. All recipients were B6 Thy1.1 mice (H-2^b). BALB.B donors were MHC-matched:mHA-mismatched, while BALB/c donors were MHC- and mHA-mismatched. Groups were as follows: (a) BALB.B LR-PLT (H-2^b; MHC-matched; mHA-mismatched); (b) BALB/c LR-PLT (H-2^d; MHC- and mHA-mismatched); (c) C57BL/6J LR-PLT (H-2^b; MHC- and mHA-matched); (d) BALB/c whole blood (H-2^d; MHC- and mHA-mismatched); (e) naive. Indicated recipients received 2 PLT transfusions followed by BMT. Seroanalyses and in vivo survival of BALB targets was also performed both prior to and after BMT (see Figures 3 and 4). (B) BMT engraftment results. Engraftment is represented by percentage of Thy1.2⁺ cells of total T cells in peripheral blood; the mean of each group is represented as a horizontal line. Statistics were generated using 1-way ANOVA with Dunnett’s post test. Combined data from 3 independent experiments are shown.