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. 2009 Aug 10;119(9):2623–2633. doi: 10.1172/JCI38660

Figure 3. Loss of PTRF is associated with deficiency and mislocalization of caveolins in muscle.

Figure 3

(A) In control muscle, PTRF was clearly seen in sarcolemma as strongly staining blood vessels. Caveolin-3 (Cav3) was clearly visible at sarcolemma, and caveion-1 and -2 stained intramuscular blood vessels. The muscle of P4 was negative for PTRF. Membrane staining of caveolin-3 was reduced with increased cytoplasmic staining, and caveolin-1 and -2 were barely detectable. Immunoreactivity of nNOS varied between muscle fibers, but was not markedly different between control and patient muscle. Scale bar: 50 μm. (B) Immunoblotting analysis of skeletal muscles. 3T3 cells were used as a positive control. PTRF and caveolin-2 were seen only in the muscles of 2 control subjects and in 3T3 cells, and were barely detectable in the muscles of P1–P5. The bands for caveolin-3 and nNOS were variably seen. (C and D) Quantification of immunoreactive bands was performed by densitometric analysis and normalized with MHC. In P1–P5, relative amounts of caveolin-3 decreased compared with control subjects (C), whereas nNOS amounts varied (D).