Differences between amounts of mucus released in luminal perfusates are shown before (basal) and after stimulation with PGE2 10–6 M (n = 6, P < 0.001) (A), carbachol 10–4 M (n = 5, P = 0.024) (B), and 5-HT 10–5 M (n = 6, P = 0.03) (C). These agonists significantly increased mucus release. On the other hand, isoproterenol 10–5 M did not stimulate mucus release (n = 4, P = 0.9) (D). Data shown are mean ± SEM.